Journal
BIORESOURCE TECHNOLOGY
Volume 145, Issue -, Pages 345-350Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.12.038
Keywords
Porphyridium sp.; Exopolysaccharide; Diafiltration
Funding
- ANR French Agency for Research
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In this work the extraction of EPSs from culture media of Porphyridium cruentum, by dialysis, solvent-precipitation with 3 polar alcohols (methanol, ethanol and isopropanol) and membrane separation techniques has been studied. Diafiltration (DF) using a membrane with a 300 kDa molecular weight cut off was the most efficient technique compared to solvent-extraction and dialysis methods. After extraction, EPS fraction was characterized in terms of rheological properties and biochemical content. The product exhibited shear thinning behavior and a critical overlap concentration equal to 0.6 g/L The monosaccharide composition was investigated after acidic hydrolysis. Xylose, galactose, glucose and glucuronic acid were identified as the main constitutive monomers. (C) 2012 Elsevier Ltd. All rights reserved.
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