Journal
BIORESOURCE TECHNOLOGY
Volume 135, Issue -, Pages 304-308Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.10.024
Keywords
Biodiesel; Cryptococcus curvatus; Sludge; Hydrodynamic cavitation; Pretreatment
Funding
- Advanced Biomass R&D Center (ABC) of Korea Grant [2011-0031348]
- Ministry of Science, Korea Minister of Ministry of Land, Transport and Maritime Affairs (MLTM) as U-city Master and Doctor Course Grant Program
- Technology of Korean government of the Republic of Korea
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Oleaginous yeast Cryptococcus curvatus has become some of the most promising feedstock for biodiesel production due to their high production efficiency. However, high cost of cultivation, especially substrate cost, hinders rapid commercialization of yeast-based biodiesel. In this study, waste activated sludge (WAS), which is rich in nutrients and organic matters, was examined as an economic substitute for organic substances. To be efficiently bioavailable, WAS must be pretreated. Hydrodynamic cavitation reaction time and pH were identified as experimental factors, whereas growth rate was selected as response parameter. The experimental factors were optimized employing Response Surface Methodology (RSM). Growth rate of 1.13/h was optimized at reaction time 12.5 min and pH 8.65. When sludge pretreated under these optimal conditions was used as a substitute to yeast extract, 9.84 g/L of biomass was obtained in a day and lipid was accumulated up to 23% of dry weight. (C) 2012 Elsevier Ltd. All rights reserved.
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