Journal
BIORESOURCE TECHNOLOGY
Volume 145, Issue -, Pages 108-115Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.03.142
Keywords
Random mutagenesis; Chlamydomonas reinhardtii; Comparative proteomics; Microalgal lipid biosynthesis
Funding
- Advanced Biomass R&D Center (ABC) of Korea
- Ministry of Education, Science and Technology [ABC-2010-0029728]
- Industrial Technology Research Infrastructure Program [N000900003]
- Ministry of Knowledge Economy (MKE, Korea)
- Chonbuk National University
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In this study, proteomic approach was employed to advance our understanding of genes associated with microalgal lipid production. We first generated random mutants of Chlamydomonas reinhardtii, which displayed increased or decreased lipid content, compared to that in the wild-type. Mutants were designated as CR12 and CR48. Then, the changes of protein expression associated with the mutations of Chlamydomonas reinhardtii were analyzed. We could identify proteins that were significantly up-regulated or down-regulated either in the wild-type or CR12 or CR48. Our results will help understand additional genes or pathways directly or indirectly linked to microalgal lipid production. (C) 2013 Elsevier Ltd. All rights reserved.
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