Journal
BIORESOURCE TECHNOLOGY
Volume 135, Issue -, Pages 39-45Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.10.095
Keywords
Switchgrass biomass; Fungal pretreatment; Solid state fermentation; Enzymatic hydrolysis; Useful enzyme production
Funding
- HATCH funds
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This study investigates fungal pretreatment of switchgrass involving solid state fermentation (SSF) to improve saccharification and simultaneously produce enzymes as co-products. The results revealed that the fungus Pycnoporus sp. SYBC-L3 can significantly degrade lignin and enhance enzymatic hydrolysis efficiency. After a 36-d cultivation period, nearly 30% reduction in lignin content was obtained without significant loss of cellulose and hemicellulose, while a considerable amount of laccase, as high as 6.3 U/g, was produced. After pretreatment, pores on switchgrass surface were observed using scanning electron microscopy (SEM). The enzymatic hydrolysis efficiency for the switchgrass with 36-d pretreatment was about 50% greater than the untreated one. Our results suggest that solid state fungal cultivation may be a good method for switchgrass pretreatment, which can simultaneously achieve high efficiency of enzymatic hydrolysis and production of some useful enzymes for other industrial utilization. (C) 2012 Elsevier Ltd. All rights reserved.
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