Journal
BIORESOURCE TECHNOLOGY
Volume 134, Issue -, Pages 190-197Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.12.057
Keywords
Denitrification; Expanded granular sludge bed (EGSB) reactor; High-nitrate waste; 454-Pyrosequencing
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Denitrification of high concentration of nitrate wastewater was investigated in expanded granular sludge bed (EGSB) reactor with sodium acetate as the carbon source. The optimal parameters were achieved with C/N mole ratio of 2.0, liquid up-flow velocity (V-up) of 3.0 m/h and pH of 6.2-8.2. Complete denitrification can be achieved even with nitrate nitrogen concentration as high as 14000 mg/L. Furthermore, 454-pyrosequencing technology was used to analyze bacterial diversity. Results showed that a total of 5573 sequences were obtained which could be affiliated to 6 phylogenetic groups, including Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes, Chloroflexi and unclassified phylum. Proteobacteria (84.53%) was the dominant microbial population, followed by Firmicutes (13.24%) and Actinobacteria (0.38%). The dominate phylum was different from that in other anaerobic system. (C) 2012 Published by Elsevier Ltd.
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