Optimization of hydrolysis conditions of Palmaria palmata to enhance R-phycoerythrin extraction

In this study, response surface methodology was applied to optimize R-phycoerythrin extraction from the red seaweed Palmaria palmata, using enzymatic digestion. Several algal treatments prior to digestion were first investigated. The extraction yield and the purity index of R-phycoerythrin, and the recovery of proteins and reducing sugars in the water-soluble fraction were then studied in relation to the hydrolysis time, the temperature and the enzyme/seaweed ratio. Enzymatic digestion appears to be an effective treatment for R-phycoerythrin extraction. Moreover, using the seaweed roughly cut in its wet form gives the most interesting results in terms of extract quality and economic cost. The R-phycoerythrin extraction yield is 62 times greater than without enzyme treatment and 16 times greater than without optimization. Enzymatic optimization enhanced the purity index up to 16 times. (C) 2012 Elsevier Ltd. All rights reserved.