Journal
WEED SCIENCE
Volume 66, Issue 2, Pages 149-158Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/wsc.2017.68
Keywords
Molecular assay; PPO inhibitors; whole-plant screen
Categories
Funding
- Arkansas Soybean Promotion Board
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Palmer amaranth is one of the most problematic weeds in the midsouthern United States, and the evolution of resistance to protoporphyrinogen oxidase (PPO) inhibitors in biotypes already resistant to glyphosate and acetolactate synthase (ALS) inhibitors is a major cause of concern to soybean and cotton growers in these states. A late-season weed-escape survey was conducted in the major row crop-producing counties (29 counties) to determine the severity of PPO-inhibitor resistance in Arkansas. A total of 227 Palmer amaranth accessions were sprayed with fomesafen at 395 g ha(-1) to identify putative resistant plants. A TaqMan qPCR assay was used to confirm the presence of the Delta G210 codon deletion or the R128G/M (homologous to R98 mutation in common ragweed) target-site resistance mechanisms in the PPX2 gene. Out of the 227 accessions screened, 44 were completely controlled with fomesafen, and 16 had only one or two severely injured plants (>= 98% mortality) when compared with the 1986 susceptible check (100% mortality). The remaining 167 accessions were genotypically screened, and 82 (49%) accessions were found to harbor the Delta G210 deletion in the PPX2 gene. The R128G was observed in 47 (28%) out of the 167 accessions screened. The mutation R128M, on the other hand was rare, found in only three accessions. About 13% of the accessions were segregating for both the Delta G210 and R128G mutations. Sixteen percent of the tested accessions had mortality ratings < 90% and did not test positive for the Delta G210 or the R128G/M resistance mechanisms, indicating that a novel target or non-target site resistance mechanism is likely. Overall, PPO inhibitor-resistant Palmer amaranth is widespread in Arkansas, and the Delta G210 resistance mechanism is especially dominant in the northeast corridor, while the R128G mutation is more prevalent in counties near Memphis, TN.
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