Journal
BIORESOURCE TECHNOLOGY
Volume 137, Issue -, Pages 9-17Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.03.109
Keywords
Biohydrogen production; Response surface methodology (RSM); Hydrogenase; Medium compositions; Klebsiella pneumoniae
Funding
- National Special Fund of China for State Key Laboratory of Bioreactor Engineering [2060204]
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The low yield of the biohydrogen production is the main constraint for its industrialization process. In order to improve its production, medium compositions of the hydrogen fermentation by Klebsiella pneumoniae ECU-15 were optimized through the response surface methodology (RSM). Experimental results showed that the optimum hydrogen production of 5363.8 ml/L was obtained when the concentration of glucose, the ammonium sulfate and the trace elements were 35.62 g/L, 2.78 g/L and 23.15 ml/L at temperature 37.0 degrees C, pH 6.0. H-2 evolving hydrogenase was greatly enhanced by the optimization of the medium compositions. The activity of H-2 evolving hydrogenase increased with the temperature, and decreased with the pH, while the activity of the uptake hydrogenase increased with the temperature and the pH. So the biohydrogen production process of the K. pneumoniae ECU-15 was the comprehensive results of the evolution hydrogen process and the uptake hydrogen process. (C) 2013 Elsevier Ltd. All rights reserved.
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