Journal
BIORESOURCE TECHNOLOGY
Volume 135, Issue -, Pages 481-489Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.09.063
Keywords
Lactic acid fermentation; Corn stover; Simultaneous saccharification and fermentation (SSF); Pediococcus acidilactici DQ2; High lactic acid titer
Funding
- National Basic Research Program of China [2011CB707406]
- National High-Tech Program of China [2012AA022301]
- Natural Science Foundation of China [20976051]
- China Postdoctoral Science Foundation [2011M500742/2012T50380]
- Central Universities of China [WF0913005/WF1114054]
- Shanghai Leading Academic Discipline Project [B505]
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lactic acid bacterium with high tolerance of temperature and lignocellulose derived inhibitor was isolated and characterized as Pediococcus acidilactici DQ2. The strain used in the simultaneous saccharification and fermentation (SSF) for high titer lactic acid production at the high solids loading of corn stover. Corn stover was pretreated using the dry sulphuric acid pretreatment, followed by a biological detoxification to remove the inhibitors produced in the pretreatment. The bioreactor with a novel helical impeller was used to the SSF operation of the pretreated and biodetoxified corn stover. The results show that a typical SSF operation at 48 degrees C, pH 5.5, and near 30% (w/w) solids loading in both 5 and 50 L bioreactors was demonstrated. The lactic acid titer, yield, and productivity reached 101.9 g/L, 77.2%, and 1.06 g/L/h, respectively. The result provided a practical process option for cellulosic lactic acid production using virgin agriculture lignocellulose residues. (C) 2012 Elsevier Ltd. All rights reserved.
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