Journal
BIORESOURCE TECHNOLOGY
Volume 148, Issue -, Pages 114-120Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.08.101
Keywords
Lipase; Solvent tolerant; Cold-active; Stenotrophomonas maltophilia; Non-aqueous
Funding
- National Basic Research Program of China (973 Program) [2011CB710800]
- Hi-Tech Research and Development Program of China (863 Program) [2011AA02A209]
- National Natural Science Foundation of China [20936002]
- Zhejiang University Foundation [2013QNA4034]
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An extracellular organic solvent-tolerant and cold-active lipase producing bacterium was isolated from oil-contaminated soil samples, and identified taxonomically as Stenotrophomonas maltophilia. The lipase from S. maltophilia CGMCC 4254 (SML) was purified 60.5-fold to homogeneity with 38.9 U/mg specific activity. Partially purified SML displayed remarkable stability in 50% and 100% (v/v) hydrophobic organic solvents after incubation for 7 days. The enzyme also retained more than 50% of its residual activity in several pure hydrophilic organic solvents after incubation for 7 days. SML showed 57% maximum activity at 5 degrees C, and had optimal activity at 35 degrees C. These unique properties of SML make it promising as a biocatalyst for industrial processes. (C) 2013 Elsevier Ltd. All rights reserved.
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