Journal
BIORESOURCE TECHNOLOGY
Volume 127, Issue -, Pages 407-414Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.09.130
Keywords
Recombinant chitosanase; Bacillus subtilis; Secretion; Chitin waste; Chitosan-oligosaccharide production
Funding
- National Research Council of Thailand (NRCT)
- Suranaree University of Technology (SUT)
- Thailand Research Fund (TRF-MAG) [525S067]
- Higher Education Research Promotion
- National Research University Project of Thailand, Office of the Higher Education Commission
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Chitosanases are enzymes that catalyse the hydrolysis of the beta-1,4 glycosidic bond of chitosan. One of the most promising applications of this enzyme is for the bioconversion of chitosan into value-added chitosan-oligosaccharides (COS). GH46 chitosanase (Csn) from Bacillus subtilis 168 was expressed in Escherichia coli by fusing the gene encoding mature Csn to the E. coli OmpA signal peptide sequence. The recombinant enzyme was secreted into the culture supernatant. The recombinant Csn showed high specific activity and stability over a wide range of pH. The enzyme was > 100 times more thermostable in the presence of the substrate, with a half-life time of activity (tau(1/2)) of approximately 20 h at 50 degrees C and pH 5.5. Efficient bioconversion of chitosan into different mixtures of COS, using crude culture supernatant containing secreted enzyme was demonstrated. (c) 2012 Elsevier Ltd. All rights reserved.
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