Journal
BIORESOURCE TECHNOLOGY
Volume 142, Issue -, Pages 225-231Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.05.027
Keywords
Pseudomonas putida; Polyhydroxyalkanoate; Block copolymer; Random copolymer
Funding
- State Basic Science Foundation 973 [2012CB725201]
- 863 project [2012AA02A702]
- State Industrialization Grant (Zhicheng Grant) [2012BAD32B02]
- China National Nature Science Foundation [31170099]
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The beta-oxidation weakened Pseudomonas putida were established as a platform for the production of polyhydroxyalkanoates (PHA) with adjustable monomer compositions and micro-structures. When mutant P. putida KTOYO6 Delta C (phaPCJ(A.c)) was cultivated on mixtures of sodium butyrate and sodium hexanoate (C4:C6), random copolymers of P(3HB-co-3HHx) consisting of 3-hydroxybutyrate (3HB), 3-hydroxyhexanoate (3HHx), were accumulated with 3HHx content ranged from 19 mol% to 75 mol%. While recombinant P. putida KTQQ20 grown on mixtures of sodium hexanoate and decanoic acid (C6:C10), produced random copolymers of P(3HHx-co-3HD) consisting of 3-hydroxyhexanoate (3HHx) and 3-hydroxydecanoate (3HD), the monomer fraction of 3HHx ranged from 16 mol% to 63 mol%. The comonomer compositions were easily regulated by varying the fatty acid concentrations. P. putida KTQQ20 produced a novel diblock copolymer P3HHx-b-P(3HD-co-3HDD) consisting of 49 mol% P3HHx and 51 mol% P(3HD-co-3HDD) [35.25 mol% 3HDD (3-hydroxydodecanoate)], which was characterized by C-13 NMR, HMBC NMR, DSC, GPC and universal testing machine. (C) 2013 Elsevier Ltd. All rights reserved.
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