Journal
BIORESOURCE TECHNOLOGY
Volume 137, Issue -, Pages 254-260Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.03.084
Keywords
Butanol; Clostridium beijerinckii; Low-energy ion implantation; N-methyl-N-nitro-N-nitrosoguanidine mutation; Solvent tolerance
Funding
- National Natural Science Foundation of China [21176105]
- Fundamental Research Funds for the Central Universities [JUSRP111A24]
- Program of the Key Laboratory of Industrial Biotechnology, Ministry of Education, China [KLIB- KF201105]
- 111 Project [111-2-06]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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In order to obtain mutant strains showing higher solvent tolerance and butanol production than those of wild-type strains, the butanol-producing strain Clostridium beijerinckii L175 was subjected to mutagenesis using a combined method of low-energy ion beam implantation and N-methyl-N-nitro-N-nitrosoguanidine induction. With this effort, mutant strain MUT3 was isolated. When it was used for butanol fermentation in P2 medium, the production of butanol was 15.8 +/- 0.7 g/L 46% higher than the wild-type strain. Furthermore, after optimization of butanol production from cane molasses with MUT3, the maximum butanol production of 14.9 +/- 0.5 g/L were obtained in crew-capped bottles. When ABE production by MUT3 was carried out in a bioreactor, the production of butanol and total solvent were 15.1 +/- 0.8 g/L and 22.1 +/- 0.9 g/L, respectively. The remarkable butanol production and solvent tolerance of MUT3 make it promising for butanol production from cane molasses. (C) 2013 Elsevier Ltd. All rights reserved.
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