Journal
BIORESOURCE TECHNOLOGY
Volume 127, Issue -, Pages 386-390Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.09.068
Keywords
Tetraselmis subcordiformis; Biodiesel; BODIPY 505/515; Lipid determination; Nitrogen limitation
Funding
- Shandong Science and Technology plan Project [2011GHY11528]
- Hi-Tech Research and Development Program (863) of China [2012AA052103]
- Specialized Fund for the Basic Research Operating expenses Program [20603022012004, 2010-ts-03]
- National Natural Science Foundation of China [41176153]
- Natural Science Foundation of Shandong Province [2009ZRA02075]
- Qingdao Municipal Science and Technology plan project [11-3-1-5-hy]
- National Marine Public Welfare Research Project [200805069]
- National Science & Technology Pillar Program [2008BAD95B11, 2010BAC68B03]
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BODIPY 505/515, a lipophilic bright green fluorescent dye was tested for lipid detection in the microalga Tetraselmis subcordiformis. A concentration of 0.28 mu g ml(-1) and staining for 6 min was optimal. Lipid bodies stained with BODIPY505/515 had a characteristic green fluorescence. Their volumes were determined using the sphere volume formula. Lipid accumulation under different nitrogen concentrations was analyzed. With an increase in NaNO3 concentration from 0 to 240 mg L-1, the maximum algal concentration increased from 8.23 +/- 0.62 (x 10(5) cells ml(-1)) to 1.61 +/- 0.13 (x 10(6) cells ml(-1)), while the maximum volume of intracellular neutral lipid decreased from 9.78 +/- 1.77 mu m(3) cell(-1) to 6.00 +/- 0.59 mu m(3) cell(-1). A comparison of the lipid contents measured by BODIPY 505/515 staining and the gravimetric method showed a positive correlation coefficient of R-2 = 0.93. BODIPY 505/515 staining is a promising method in lipid quantitation in T. subcordiformis. (c) 2012 Elsevier Ltd. All rights reserved.
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