Journal
BIORESOURCE TECHNOLOGY
Volume 144, Issue -, Pages 623-631Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.07.018
Keywords
Co-culture; Mild alkali pretreatment; Sugarcane bagasse; Bio-hydrogen
Funding
- National Natural Science Foundation of China [51078147, 51278200]
- Guangdong Natural Science Foundation [S2012010010380]
- Fundamental Research Funds for the Central Universities, SCUT [2012ZM0081]
- Guangdong Provincial Science and Technology Program [2012B091100163, 2011B090400033]
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A novel co-culture of Clostridium thermocellum and Thermoanaerobacterium aotearoense with pretreated sugarcane bagasse (SCB) under mild alkali conditions for bio-hydrogen production was established, exhibiting a cost-effective and synergetic advantage in bio-hydrogen production over monoculture of C. thermocellum or T. aotearoense with untreated SCB. The optimized pretreatment conditions were established to be 3% NaOH, and a liquid to solid ratio of 25:1 at 80 degrees C for 3 h. A final hydrogen production of 50.05 +/- 1.51 mmol/L was achieved with 40 g/L pretreated SCB at 55 degrees C. The established co-culture system provides a novel consolidated bio-processing strategy for bioconversion of SCB to bio-hydrogen. (C) 2013 Elsevier Ltd. All rights reserved.
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