Journal
BIORESOURCE TECHNOLOGY
Volume 147, Issue -, Pages 240-245Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.08.037
Keywords
Chlorella sp KR-1; Dimethyl carbonate; Fatty acid methyl esters; Microalgae; Novozyme 435
Funding
- Marine Biotechnology Program
- Ministry of Oceans and Fisheries, Korea
- Advanced Biomass R&D Center (ABC) of Global Frontier Project
- Ministry of Education, Science and Technology [ABC-2012-053880]
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We developed a method for the highly efficient lipid extraction and lipase-catalyzed transesterification of triglyceride from Chlorella sp. KR-1 using dimethyl carbonate (DMC). Almost all of the total lipids, approximately 38.9% (w/w) of microalgae dry weight, were extracted from the dried microalgae biomass using a DMC and methanol mixture (7:3 (v/v)). The extracted triglycerides were transesterified into fatty acid methyl esters (FAMES) using Novozyme 435 as the biocatalyst in DMC. Herein, DMC was used as the reaction medium and acyl acceptor. The reaction conditions were optimized and the FAMEs yield was 293.82 mg FAMEs/g biomass in 6 h of reaction time at 60 degrees C in the presence of 0.2% (v/v) water. Novozyme 435 was reused more than ten times while maintaining relative FAMEs conversion that was greater than 90% of the initial FAMEs conversion. (C) 2013 Elsevier Ltd. All rights reserved.
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