Journal
BIORESOURCE TECHNOLOGY
Volume 130, Issue -, Pages 517-521Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.12.106
Keywords
Cofactor regeneration; Azoreductase; Glucose 1-dehydrogenase; Dye removal; Methyl red
Funding
- Science and Technology Project of Zhejiang Province [2010C13G2010074, 2008C13014-3]
- National Natural Science Foundation of China [31070079]
- International Cooperation Project in Science and Technology of Zhejiang Province [2008C14038]
- National Key Technology R and D Program [2012BAC17B04]
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Azo dyes are toxic and carcinogenic and are often present in industrial effluents. In this research, azoreductase and glucose 1-dehydrogenase were coupled for both continuous generation of the cofactor NADH and azo dye removal. The results show that 85% maximum relative activity of azoreductase in an integrated enzyme system was obtained at the conditions: 1 U azoreductase:10 U glucose 1-dehydrogenase, 250 mM glucose, 1.0 mM NAD(+) and 150 mu M methyl red. Sensitivity analysis of the factors in the enzyme system affecting dye removal examined by an artificial neural network model shows that the relative importance of enzyme ratio between azoreductase and glucose 1-dehydrogenase was 22%, followed by dye concentration (27%), NAD(+) concentration (23%) and glucose concentration (22%), indicating none of the variables could be ignored in the enzyme system. Batch results show that the enzyme system has application potential for dye removal. (C) 2012 Elsevier Ltd. All rights reserved.
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