Journal
BIORESOURCE TECHNOLOGY
Volume 138, Issue -, Pages 55-62Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2013.03.159
Keywords
Clostridium acetobutylicum; Metabolism; Butanol
Funding
- IIT Gandhinagar, Department of Science & Technology (DST)
- IYBA, Department of Biotechnology (DBT), Government of India
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Clostridium acetobutylicum exhibits a two-step metabolic pathway where substrates are first converted to organic acids accompanied by a decrease in pH. The acids are then assimilated to organic solvents. The transition from the acid-producing (acidogenesis) to the solvent-producing phase (solventogenesis) is controlled by integration of a number of cellular and environmental cues, whose precise mode of action are not well understood. In this study, a series of batch experiments were performed to understand the impact of extracellular cues in regulating the dynamics of acidogenesis and solventogenesis. It is demonstrated that the two phases operate independently of each other and the growth phase of the cell, i.e. the cues controlling a phase are not linked to the status of the other phase or the growth phase of the cell. Kinetic experiments demonstrated that there exist two previously uncharacterized negative feedback loops controlling the amounts of acids produced in the acidogenesis phase. (C) 2013 Elsevier Ltd. All rights reserved.
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