Journal
BIORESOURCE TECHNOLOGY
Volume 114, Issue -, Pages 735-739Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.03.058
Keywords
Laccase isoforms; Sugarcane bagasse; Solid state fermentation; Pleurotus ostreatus; Zymogram
Funding
- Coordination for the Improvement of Higher Education Personnel (CAPES), within the Ministry of Education in Brazil
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Laccases are oxidative enzymes linked to biological degradation of lignin. The aim of this work was to evaluate the effect of inducers and different concentrations of nitrogen on production level of total laccase activity and pattern of laccase isoforms, produced in solid state fermentation of sugarcane bagasse by a selected strain of Pleurotus streams. The addition of yeast extract 5 g/L, copper sulfate 150 mu M and ferulic acid 2 mM provided highest enzymatic activity (167 U/g) and zymograms indicated the presence of six laccase isoforms (POXA1b, POXA3, POXC and three other isoforms). Results of protein identification by mass spectrometry confirmed the presence of POXC and POXA3 as the main isoenzymes, and also identified a glyoxal oxidase and three galactose oxidases. The fact that the isoenzyme POXA1b was not identified in the analyzed samples can be possibly explained by its sensitivity to protease degradation. (C) 2012 Elsevier Ltd. All rights reserved.
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