Journal
BIORESOURCE TECHNOLOGY
Volume 104, Issue -, Pages 715-721Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.10.076
Keywords
Organosolv pretreatment; Horticultural waste; Enzymatic hydrolysis; Saccharomyces cerevisiae; Bioethanol
Funding
- Singapore Totalisation Board
- Ngee Ann Kongsi
- Science and Engineering Research Council of the Agency for Science, Technology and Research (A*STAR), Singapore
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In this study, we investigated the use of horticultural waste (HW) collected in Singapore as a renewable raw material for bioethanol production. A modified organosolv method using ethanol cooking under mild conditions followed by H2O2 post-treatment was investigated for HW pretreatment. It was found that the addition of acid catalysts in the pretreatment process was not critical and post-treatment using H2O2 was essential for the enhancement of HW digestibility. Enzymatic hydrolysis of the organosolv pretreated HW with 17.5% solid content, enzyme loading of 20 FPU/g HW of filter paper cellulase, and 80 CBU/g HW of beta-glucosidase resulted in a HW hydrolysate containing 26.9 g/L reducing sugar after 72 h. Fermentation of the above hydrolysate medium produced 11.69 g/L ethanol at 8 h using Saccharomyces cerevisiae. It proved that horticultural waste was a potential feedstock for fuel ethanol production and organosolv pretreatment method developed in this study was effective. (C) 2011 Elsevier Ltd. All rights reserved.
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