Journal
BIORESOURCE TECHNOLOGY
Volume 126, Issue -, Pages 307-313Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.09.059
Keywords
Chlorella pyrenoidosa; Protein; Extraction; Hydrolysis
Funding
- Scientific and Technological Research and Development Program of Guangdong Province [2009B090300271]
- Scientific and Technological Research Program of Guangzhou City [2012J4100123]
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In this study, for the first time, the applications of two new methods, ionic liquid and low-temperature high-pressure cell breakage methods, to the extraction of whole proteins in Chlorella pyrenoidosa cells were explored. Meanwhile, the comparison with three traditional methods was also made. The results indicated that the extraction rate for ionic liquid is only at moderate level, but the new low-temperature high-pressure cell breakage method can obviously increase the protein extraction rate up to 2- to 15-fold. Subsequently, the hydrolysis of the extracted proteins was conducted with three enzymes (papain, trypsin and alcalase). The data presented that the degree of hydrolysis for each enzyme under the optimal conditions is in the order of: alcalase (18.31%) > papain (14.33%) > trypsin (8.47%), demonstrating the potential of C pyrenoidosa protein hydrolysates obtained here in nutritional supplement and medical foods. (C) 2012 Elsevier Ltd. All rights reserved.
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