Journal
BIORESOURCE TECHNOLOGY
Volume 115, Issue -, Pages 21-26Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.11.015
Keywords
Immobilization; Laccase; Magnetic mesoporous carbon; Phenol removal
Funding
- Fundamental Research Funds for the Central Universities, Hunan University
- Program for Changjiang Scholars and Innovative Research Team in University [IRT0719]
- National Natural Science Foundation of China [50978088, 51039001, 50608029]
- Hunan Key Scientific Research Project [2009FJ1010]
- Hunan Provincial Natural Science Foundation of China [10JJ7005]
- Hunan Provincial Innovation Foundation For Postgraduate [CX2009B080, CX2010B157]
- Program for New Century Excellent Talents in University [NCET-08-0181]
- Hunan University Graduate Education Innovation Project [531107011019]
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A novel magnetically separable laccase immobilized system was constructed by adsorbing laccase into bimodal carbon-based mesoporous magnetic composites (CMMC). A large adsorption capacity (491.7 mg g(-1)), excellent activity recovery (91.0%) and broader pH and temperature profiles than free laccase have been exhibited by the immobilized laccase. Thermal stability was enhanced to a great extent and operational stability was increased to a certain extent. The shift of kinetic parameters indicated affinity change between enzyme and substrate. Application of the immobilized system in phenol and p-chlorophenol removal was investigated in a batch system. Adsorption effects of the support were responsible for the quick removal rate in the first hour, and up to 78% and 84% of phenol and p-chlorophenol were removed in the end of the reaction, respectively, indicating that the magnetic bimodal mesoporous carbon is a promising carrier for both immobilization of laccase and further application in phenol removal. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.
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