Journal
BIORESOURCE TECHNOLOGY
Volume 119, Issue -, Pages 114-122Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.05.112
Keywords
Enzymatic deconstruction; Laccase; Bioethanol; Lignocellulose; 2D NMR
Funding
- LIGNODECO EU-project on Optimised pre-treatment of fast growing woody and nonwoody Brazilian crops [KBBE-3-244362]
- ELLE, LIGNOCELL
- RAPERO Spanish MICINN
- FEDER [AGL2008-00709, AGL2011-25379, BIO2008-01533,]
- European Social Fund (ESF)
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The ability of Trametes villosa laccase, in conjuction with 1-hydroxybenzotriazole (HBT) as mediator and alkaline extraction, to remove lignin was demonstrated during treatment of wood (Eucalyptus globulus) and non-wood (Pennisetum purpureum) feedstocks. At 50 U g(-1) laccase and 2.5% HBT concentration, 48% and 32% of the Eucalyptus and Pennisetum lignin were removed, respectively. Two-dimensional nuclear magnetic resonance of the feedstocks, swollen in dimethylsulfoxide-d(6), revealed the removal of p-hydroxyphenyl, guaiacyl and syringyl lignin units and aliphatic (mainly beta-O-4'-linked) side-chains of lignin, and a moderate removal of p-coumaric acid (present in Pennisetum) without a substantial change in polysaccharide cross-signals. The enzymatic pretreatment (at 25 U g-1) of Eucalyptus and Pennisetum feedstocks increased the glucose (by 61% and 12% in 72 h) and ethanol (by 4 and 2 g L-1 in 17 h) yields from both lignocellulosic materials, respectively, as compared to those without enzyme treatment. (C) 2012 Elsevier Ltd. All rights reserved.
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