Journal
BIORESOURCE TECHNOLOGY
Volume 118, Issue -, Pages 553-562Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.05.079
Keywords
Fermentation; Platform chemical; Organic acid; Cell adsorption; Polyethylenimine
Funding
- Swedish Governmental Agency for Innovation Systems (Vinnova)
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Propionic acid production from glycerol was studied using Propionibacterium acidipropionici DSM 4900 cells immobilized on polyethylenimine-treated Poraver (PEI-Poraver) and Luffa (PEI-Luffa), respectively. Using PEI-Luffa, the average productivity, yield and concentration of propionic acid from 40 g L-1 glycerol were 0.29 g L-1 h(-1), 0.74 mol(PA) mol(Gly)(-1) and 20 g L-1, respectively, after four consecutive recycle-batches. PEI-Poraver supported attachment of 31 times higher amounts of cells than PEI-Luffa and produced 20.28 and 35 g L-1 propionic acid from 40, 65 and 85 g L-1 glycerol, respectively (0.61 mol(PA) mol(Gly)(-1)). The corresponding production rates were 0.86, 0.43 and 0.35 g L-1 h(-1), which are the highest reported from glycerol via batch or fed-batch fermentations for equivalent propionic acid concentrations. Using a continuous mode of operation at a dilution rate of 0.1 h(-1), cell washout was observed in the bioreactor with free cells: however, propionic acid productivity, yield and concentration were 1.40 g L-1 h(-1), 0.86 mol(PA) mol(Gly)(-1), and 15 g respectively, using immobilized cells in the PEI-Poraver bioreactor. The choice of the immobilization matrix can thus significantly influence the fermentation efficiency and profile. The bioreactor using cells immobilized on PEI-Poraver allowed the fermentation of higher glycerol concentrations and provided stable and higher fermentation rates than that using free cells or the cells immobilized on PEI-Luffa. (C) 2012 Elsevier Ltd. All rights reserved.
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