Journal
BIORESOURCE TECHNOLOGY
Volume 115, Issue -, Pages 244-248Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.08.065
Keywords
D-xylonic acid; D-xylose; High-value chemical; E. coli
Funding
- Priority Research Centers Program through the National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [2010-0028300]
Ask authors/readers for more resources
An engineered Escherichia coli was constructed to produce D-xylonic acid, one of the top 30 high-value chemicals identified by US Department of Energy. The native pathway for D-xylose catabolism in E. coli W3110 was blocked by disrupting xylose isomerase (XI) and xylulose kinase (XK) genes. The native pathway for xylonic acid catabolism was also blocked by disrupting two genes both encoding xylonic acid dehydratase (yagE and yjhG). Through the introduction of a D-xylose dehydrogenase from Caulobacter crescentus, a D-xylonic acid producing E. coli was constructed. The recombinant E. coli produced up to 39.2 g L-1 D-xylonic acid from 40 g L-1 D-xylose in M9 minimal medium. The average productivity was as high as 1.09 g L-1 h(-1) and no gluconic acid byproduct was produced. These results suggest that the engineered E. coli has a promising application for the industrial-scale production of D-xylonic acid. (C) 2011 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available