Journal
BIORESOURCE TECHNOLOGY
Volume 117, Issue -, Pages 92-98Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.04.005
Keywords
Xylose; Xylulose; Simultaneous isomerization and fermentation; Hollow fiber; Saccharomyces cerevisiae
Funding
- Ohio Third Frontier Advanced Energy Program [AEP 08-044]
- Department of Energy Office of Biomass Program [GO-18163]
- National Science Foundation CBET Division [0933250, 0959920]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [0959920, 0933250] Funding Source: National Science Foundation
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A system that incorporates a packed bed reactor for isomerization of xylose and a hollow fiber membrane fermentor (HFMF) for sugar fermentation by yeast was developed for facile recovery of the xylose isomerase enzyme pellets and reuse of the cartridge loaded with yeast. Fermentation of pre-isomerized poplar hydrolysate produced using ionic liquid pretreatment in HFMF resulted in ethanol yields equivalent to that of model sugar mixtures of xylose and glucose. By recirculating model sugar mixtures containing partially isomerized xylose through the packed bed and the HFMF connected in series, 39 g/l ethanol was produced within 10 h with 86.4% xylose utilization. The modular nature of this configuration has the potential for easy scale-up of the simultaneous isomerization and fermentation process without significant capital costs. (C) 2012 Elsevier Ltd. All rights reserved.
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