Journal
BIORESOURCE TECHNOLOGY
Volume 111, Issue -, Pages 378-382Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.01.152
Keywords
Biodiesel; Enzyme-assisted extraction; Microbial lipids; Oleaginous yeast
Funding
- National Basic Research and Development Program of China (973 Program) [2011CB707405]
- Chinese Academy of Sciences [KSCX2-EW-G-1-3]
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Lipids produced by oleaginous microorganisms are a potential feedstock for biodiesel production and chemical synthesis. Yet, the costs of microbial lipids remain high, partially because the lipid recovery process is tedious and costly. In the present study, enzyme-assisted extraction of lipids from the culture of the yeast Rhodosporidium toruloides was carried out. With a heat pre-treatment with microwave, enzymatic treatment with the recombinant beta-1,3-glucomannanase, plMAN5C, and extraction with ethyl acetate, 96.6% of the total lipids were extracted from R. toruloides cells at room temperature and atmospheric pressure directly from the culture without dewatering. Therefore, this process could significantly reduce energy consumption and costs for lipids extraction from the yeast. (C) 2012 Elsevier Ltd. All rights reserved.
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