Journal
BIORESOURCE TECHNOLOGY
Volume 110, Issue -, Pages 71-78Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.01.078
Keywords
Pseudomonas; Biodegradation; Paper mill sewage sediment; Comet assay; EROD assay
Funding
- Department of Biotechnology, Government of India, and New Delhi, India
- University Grants Commission (UGC), New Delhi, Government of India
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Organic pollutants present in the pulp and paper mill sewage were extracted with dichloromethane/acetone (1:1 v/v), and the extract was added to minimal salt medium to study the degrading and detoxifying potential of Pseudomonas sp. strain ISTDF1. GC-MS analysis performed after biodegradation showed removal of chlorinated compounds like dichloroacetic acid, heptadecyl ester (RT = 28.99) and formation of simpler compounds like 4-isopropylbenzaldehyde (RT = 7.52). Methyltetrazolium (MTT) assay for cytotoxicity, 7-ethoxyresorufin-O-deethylase (EROD) assay for dioxin-like behaviour and alkaline comet assay for genotoxicity were carried out in human hepato-carcinoma cell line HuH7 before and after bacterial treatment. The bacterium reduced toxicity as shown by a 5-fold increase in LC50 value, 2110-fold reduction in benzo (alpha) pyrene equivalent value and an 8-fold reduction in Olive Tail Moment after 240 h treatment. The EROD assay was found to be a useful method which indicated positive correlation with MTT and comet assay in post-biodegradation toxicity evaluation. (C) 2012 Elsevier Ltd. All rights reserved.
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