Journal
BIORESOURCE TECHNOLOGY
Volume 111, Issue -, Pages 360-367Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.02.039
Keywords
Functional screening; Gain-of-function method; Stress-tolerance genes; Stress-tolerant fungi; Halotolerance
Funding
- Centre of excellence for integrated approaches in chemistry and biology of proteins [OP13.1.1.2.02.0005]
- European regional development fund
- Slovenian Ministry of higher education, science and technology
- Slovenian Research Agency (Infrastructural Centre Mycosmo) [J4-2022]
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Fungal species from extreme environments represent an underexploited source of stress-resistance genes. These genes have the potential to improve stress tolerance of economically important microorganisms and crops. An efficient high-throughput method for the identification of biotechnologically interesting genes of extremotolerant fungi was developed by constructing a cDNA expression library in Saccharomyces cerevisiae and screening for gain-of-function transformants under stress conditions. The advantages and possible modifications of this method are discussed, and its efficiency is demonstrated using the stress-tolerant basidiomycetous yeast Rhodotorula mucilaginosa. Twelve R. mucilaginosa genes are described that increase halotolerance in S. cerevisiae. These include genes encoding a phosphoglucomutase and a phosphomannomutase. All 12 investigated genes might be useful for the improvement of halotolerance in genetically modified crops or industrial microorganisms. (C) 2012 Elsevier Ltd. All rights reserved.
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