Journal
BIORESOURCE TECHNOLOGY
Volume 114, Issue -, Pages 414-418Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.03.025
Keywords
Sulfated rhamnan; Depolymerization; Structure; Anticoagulant activity; Heparin cofactor II
Funding
- National Natural Science Foundation of China [41076086]
- Doctoral Program of Higher Education of China [20090132110015]
- Science and Technology Development Program of Shandong Province, China [2010GHY10509, 2006GG2205014]
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A low molecular weight fraction, designated LMWP, was prepared by mild acid hydrolysis of sulfated rhamnan from Monostroma latissimum and purified by anion-exchange and gel-permeation chromatography. Chemical and spectroscopic analyses showed that LMWP was mainly composed of rhamnose, and its molecular weight was about 33.6 kDa. The backbone of LMWP consists of 1,3-linked alpha-L-rhamnose units with partially sulfate groups at the C-2 position. Approximately 25% of 1,3-linked alpha-L-rhamnose units is substituted at C-2 by sulfated or non-sulfated 1,3-linked alpha-L-rhamnose and 1,2-linked alpha-L-rhamnose units. LMWP effectively prolonged clotting time as evaluated by the activated partial thromboplastin time assay and was a potent thrombin inhibitor mediated by heparin cofactor II. The investigation demonstrated that LMWP is a novel sulfated polysaccharide with anticoagulant activity. (C) 2012 Elsevier Ltd. All rights reserved.
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