Journal
BIORESOURCE TECHNOLOGY
Volume 115, Issue -, Pages 84-87Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.09.085
Keywords
Glycolipid; Biosurfactant; Biocatalysis; Glycosylation; Cellodextrin phosphorylase
Funding
- 'Bijzonder Onderzoeksfonds' (BOF) of Ghent University [01W01908]
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Glycolipids have gained increasing attention as natural surfactants with a beneficial environmental profile. They are typically produced by fermentation, which only gives access to a limited number of structures. Here we describe the biocatalytic production of novel glycolipids with the cellodextrin phosphorylase from Clostridium stercorarium. This enzyme was found to display a broad donor and acceptor specificity, allowing the synthesis of five different products. Indeed, using either alpha-glucose 1-phosphate or alpha-galactose 1-phosphate as glycosyl donor, sophorolipid as well as glucolipid could be efficiently glycosylated. The transfer of a glucosyl moiety afforded a mixture of products that precipitated from the solution, resulting in near quantitative yields. The transfer of a galactosyl moiety, in contrast, generated a single product that remained in solution at thermodynamic equilibrium. These glycolipids not only serve as a new class of biosurfactants, but could also have applications in the pharmaceutical and nanomaterials industries. (C) 2011 Elsevier Ltd. All rights reserved.
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