Journal
BIORESOURCE TECHNOLOGY
Volume 121, Issue -, Pages 131-138Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.05.144
Keywords
Laccase; Lignin; Kraft dissolved lignin (KDL); Polymerisation; Lignin fractionation
Funding
- Xunta de Galicia [09TMT012E]
- FEDER
- Ministry of Science and Innovation [CTQ2009-13651]
- Fundacao para a Ciencia e a Tecnologia - Portugal [POP-QREN BD 42684/2008]
Ask authors/readers for more resources
The potential ability of the laccase from Myceliophthora thermophila, either alone or with low molecular weight (LMW) additives, to polymerise a dissolved lignin from Kraft liquor of eucalypt cooking was investigated. A previous study of enzymatic performance (activity and stability) was carried out using a design experiment methodology. In addition, Kraft dissolved lignin (KDL) was fractionated according to two different protocols (solvent extraction and acidic fractionation) in order to identify possible lignin fractions with noticeable polymerisation ability. KDL and its corresponding lignin fractions were treated with laccase and analysed by size exclusion chromatography and Fourier transform infrared spectroscopy. The results provide conclusive evidence of notable lignin modifications after incubation with laccase. Moreover, lignin fractionation allows to obtain lignin fractions with different chemical characteristics and polymerisation capability. Depending on the type of raw lignin, molecular weight can increase from 4- to 21-fold by means of laccase polymerisation. (C) 2012 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available