Journal
BIORESOURCE TECHNOLOGY
Volume 117, Issue -, Pages 208-213Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.04.091
Keywords
Rhamnolipids; Fed-batch fermentation; Optimization; Pseudomonas aeruginosa; Kinetics
Funding
- Natural Science Foundation of Tianjin [09JCYBJC09400]
- Natural Science Foundation of China [21076148]
- Key Technology R&D Program of Tianjin [11ZCKFSY01000]
- Program of Introducing Talents of Discipline to Universities [B06006]
- Technological Research and Development Programs of the China Offshore Environmental Services Ltd. [CY-HB-10-ZC-055]
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Rhamnolipids find broad applications as natural surfactants, emulsifiers, and antibiotics because of their low toxicity, high biodegradability and environmental soundness. In this study, a pH stage-controlled process of fermentation of rhamnolipids by Pseudomonas aeruginosa O-2-2 was established. A yield of 24.06 g/L in batch fermentation was achieved in a 5-L fermentor via the optimization of stirring speed. By controlling pH, rhamnolipid production was increased to 28.8 g/L, an improvement of 19.7%, and more substrate was converted to rhamnolipids rather than to biomass. Fermentation kinetics models for cell growth, product synthesis and substrate consumption based on the pH stage-controlled fermentation indicated that rhamnolipid production could be further improved by fed-batch fermentation. Rhamnolipid production reached 70.56 g/L, an improvement of 193%, in the pH stage-controlled fed-batch fermentation when the stirring speeds was controlled at 500 rpm and the fermentation temperature was maintained at 30 degrees C. (C) 2012 Elsevier Ltd. All rights reserved.
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