Journal
BIORESOURCE TECHNOLOGY
Volume 116, Issue -, Pages 247-251Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.11.109
Keywords
Halophiles; alpha-Amylase; Maltooligosaccharides; Marinobacter sp.; Solvent stable
Funding
- Department of Biotechnology (Government of India)
- Council of Scientific and Industrial Research (CSIR), India
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Maltooligosaccharides especially maltotriose and maltotetraose producing amylases are highly desirable for application in bread making and other food industries. A maltotriose and maltotetraose producing amylase from moderately halophilic Marinobacter sp. EMB8 is described. Under optimized culture conditions, 48.0 IU/mL amylase was obtained. The enzyme was purified to homogeneity by ultrafiltration, DEAE cellulose and Sephadex G-75 column chromatography with 52% yield and 76-fold purification. It was a monomeric protein of 72 kDa. The amylase had many novel features viz, stability up to 20% NaCl, 80 degrees C temperature, pH 6.0-11.0 and in wide range of organic solvents at high concentrations. The enzyme efficiently hydrolyzed starch into maltooligosaccharides rich in maltotriose and maltotetraose. These novel properties make the Marinobacter sp. amylase a potentially useful enzyme. (C) 2011 Elsevier Ltd. All rights reserved.
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