Journal
BIORESOURCE TECHNOLOGY
Volume 102, Issue 19, Pages 9090-9095Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.06.085
Keywords
Xylanase; Enzymatic hydrolysis; Wheat straw; Cellulase; Thermostable
Funding
- EU Commission [213139]
- Natural Science Foundation of China [30700631]
- Northwest AF University
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Recombinant xylanase preparations from Nonomuraea flexuosa (Nf Xyn, GH11) and Thermoascus aurantiacus (Ta Xyn, GH10) were evaluated for their abilities to hydrolyze hydrothermally pretreated wheat straw. The GH family 10 enzyme Ta Xyn was clearly more efficient in solubilizing xylan from pretreated wheat straw. Improvement of the hydrolysis of hydrothermally pretreated wheat straw by addition of the thermostable xylanase preparations to thermostable cellulases was evaluated. Clear synergistic enhancement of hydrolysis of cellulose was observed when cellulases were supplemented even with a low amount of pure xylanases. Xylobiose was the main hydrolysis product from xylan. It was found that the hydrolysis of cellulose increased nearly linearly with xylan removal during the enzymatic hydrolysis. The results also showed that the xylanase preparation from T. aurantiacus, belonging to GH family 10 always showed better hydrolytic capacity of solubilizing xylan and acting synergistically with thermostable cellulases in the hydrolysis of hydrothermally pretreated wheat straw. (C) 2011 Elsevier Ltd. All rights reserved.
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