Journal
BIORESOURCE TECHNOLOGY
Volume 102, Issue 2, Pages 742-747Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2010.08.074
Keywords
Lignocellulosic biomass degradation; Microbial consortium; Enrichment; Raw corn stover
Funding
- Program for New Century Excellent Talents in University [NCET-05-0338]
- National Creative Research Groups of China [50821002]
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A microbial consortium with a high cellulolytic activity was enriched to degrade raw corn stover powder (RCSP). This consortium degraded more than 51% of non-sterilized RCSP or 81% of non-sterilized filter paper within 8 days at 40 degrees C under facultative anoxic conditions. Cellulosome-like structures were observed in scanning electron micrographs (SEM) of RCSP degradation residue. The high cellulolytic activity was maintained during 40 subcultures in a medium containing cellulosic substrate. Small ribosomal gene sequence analyses showed the consortium contains uncultured and cultured bacteria with or without cellulolytic activities. Among these bacteria, some are anaerobic others aerobic. Analyses of the culture filtrate showed a typical anoxic polysaccharide fermentation during the culturing process. Reducing sugar concentration increased at early stage followed by various fermentation products that were consumed at the late stage. (C) 2010 Elsevier Ltd. All rights reserved.
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