Journal
BIORESOURCE TECHNOLOGY
Volume 102, Issue 4, Pages 3927-3933Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2010.11.115
Keywords
Biosurfactant; Crude glycerol; Glycolipids; Fed-batch fermentation; Ustilago maydis
Funding
- Singapore Economic Development Board
- Temasek Trust
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Ustilago maydis is known to produce glycolipid-type biosurfactants. Here, we show that U. maydis is able to efficiently convert biodiesel-derived crude glycerol to glycolipids. We have optimized the medium composition and environmental factors for bioconversion of crude glycerol to glycolipids. The synthetic medium (MinCG) contains 50 g L-1 crude glycerol and 20.3 mg L-1 ammonium citrate as the carbon and nitrogen sources, respectively. The supplementation of trace amount of amino acids, Group-B vitamins and precursors of glycolipids, mannose and erythritol, also improved the final yield. At pH 4.0 and 30 degrees C, 32.1 g L-1 total glycolipids was produced in a 8.2-day fed-batch bioprocess. Methanol at 2% or above severely inhibited cell growth and production of glycolipids. Our results suggest that U. maydis is an excellent host for the bioconversion of crude glycerol to value-added products. (C) 2010 Elsevier Ltd. All rights reserved.
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