Journal
BIOFABRICATION
Volume 10, Issue 3, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/1758-5090/aab004
Keywords
paraoxon biosensor; metal-enhanced fluorescence; hydrogel microarray; quantum dots; silica-coated silver nanoparticles
Funding
- National Research Foundation of Korea (NRF) - Korea government (MSIP) [NRF-2017M3D1A1039289, 2017M3A7B4041798, 2009-0093823, 2016R1D1A1B0 3932220, 2017M3A7B4049850]
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In this study, we developed an enzyme-based miniaturized fluorescence biosensor to detect paraoxon, one of the most well-known neurotoxic organophosphorus compounds. The biosensor was fabricated with poly(ethylene glycol) (PEG) hydrogel microarrays that entrapped acetylcholinesterase (AChE) and quantum dots (QDs) as fluorescence reporters. Metal-enhanced fluorescence (MEF) was utilized to amplify the fluorescence signal, which was achieved by decorating QDs on the surface of silica-coated silver nanoparticles (Ag@Silica). The MEF effects of Ag@Silica were optimized by tuning the thickness of the silica shells, and under the optimized conditions, the fluorescence intensity was shown to be increased 5 fold, compared with the system without MEF. PEG hydrogel microarray entrapping QD-decorated Ag@Silica and AChE was prepared via photopatterning process. The entrapped AChE hydrolyzed paraoxon to produce p-nitrophenol within the hydrogel microstructure, which subsequently quenched the fluorescence of the QDs on the surface of Ag@Silica. The MEF-assisted fluorescence detection resulted in a significant enhancement of paraoxon detection. The detection limit was approximately 1.0 x 10(-10) Mand 2.0 x 10(-7) M for sensing with and without MEF, respectively. The successful integration of a hydrogel microarray system with a microfluidic system was demonstrated to be a potential application for the MEF-based micro-total-analysis-system.
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