Journal
BIORESOURCE TECHNOLOGY
Volume 102, Issue 11, Pages 6600-6604Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.03.070
Keywords
Succinate; PHA; Escherichia coli; Metabolic engineering; Biorefinery
Funding
- Shandong Science and Technology Development Plan [2010GSF10202]
- National Natural Science Foundation of China [30870022]
- National Basic Research Program of China [2011CB707405]
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The strategic design of this study aimed at producing succinate and polyhydroxyalkanoate (PHA) from substrate mixture of glycerol/glucose and fatty acid in Escherichia coli. To accomplish this, an E. coli KNSP1 strain derived from E. coli LR1110 was constructed by deletions of ptsG, sdhA and pta genes and overexpression of phaC1 from Pseudomonas aeruginosa. Cultivation of E. coli KNSP1 showed that this strain was able to produce 21.07 g/L succinate and 0.54 g/L PHA (5.62 wt.% of cell dry weight) from glycerol and fatty acid mixture. The generated PHA composed of 58.7 mol% 3-hydroxyoctanoate (3HO) and 41.3 mol% 3-hydroxydecanoate (3HD). This strain would be useful for complete utilization of byproducts glycerol and fatty acid of biodiesel production process. (C) 2011 Elsevier Ltd. All rights reserved.
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