Journal
BIORESOURCE TECHNOLOGY
Volume 102, Issue 8, Pages 5229-5236Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.01.062
Keywords
Biofuel; Cellobiose; Cellulose; Ethanol; Petite mutant
Funding
- Spanish Ministerio de Ciencia e Innnovacion [BIO2007-67708-004-02]
- Federation of European Biochemical Societies (FEBS)
- CSIC
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Constructs carrying the Saccharomycopsis fibuligera beta-glucosidase gene (BGL1) under the control of a constitutive actin or a galactose-inducible promoter were introduced into eleven Saccharomyces strains. In ten of these recombinant strains, BGL1 expression driven by the actin promoter was between 1.6- and 18-fold higher than that obtained with the galactose-inducible promoter. Strains carrying the actin promoter yielded ethanol concentrations from cellobiose of between 0.5% and 14%, depending on their ability to accumulate Bgl1 (between 30 and 250 mU/mL) but also on their genetic background. Comparative analysis of a S. cerevisiae strain and its corresponding petite version showed similar ethanol yields, despite a 3-fold lower beta-glucosidase production of the latter, suggesting that respiratory activity could be one of the factors influencing ethanol production when using carbon sources other than glucose. This study provides a selection of strains that may be good candidates as hosts for ethanol biosynthesis from cellulosic substrates. (C) 2011 Elsevier Ltd. All rights reserved.
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