Journal
BIORESOURCE TECHNOLOGY
Volume 102, Issue 22, Pages 10633-10640Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.09.042
Keywords
Algae oil; Biofuel; Chlorella sorokiniana; Chlorella vulgaris; Nitrogen limitation
Funding
- Ministry of Science, Technology and Innovation (MOSTI), Malaysia [02-01-12-SF0004, 02-01-12-SF0089]
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This study was undertaken to investigate the effects of different nitrate concentrations in culture medium on oil content and fatty acid composition of Chlorella vulgaris (UMT-M1) and Chlorella sorokiniana (KS-MB2). Results showed that both species produced significant higher (p < 0.05) oil content at nitrate ranging from 0.18 to 0.66 mM with C vulgaris produced 10.20-11.34% dw, while C sorokiniana produced 15.44-17.32% dw. The major fatty acids detected include C16:0, C18:0, C18:1, C18:2 and C18:3. It is interesting to note that both species displayed differentially regulated fatty acid accumulation patterns in response to nitrate treatments at early stationary growth phase. Their potential use for biodiesel application could be enhanced by exploring the concept of binary blending of the two microalgae oils using developed mathematical equations to calculate the oil mass blending ratio and simultaneously estimated the weight percentage (wt.%) of desirable fatty acid compositions. (C) 2011 Elsevier Ltd. All rights reserved.
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