Journal
BIORESOURCE TECHNOLOGY
Volume 102, Issue 19, Pages 8921-8929Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.07.047
Keywords
(Woody) Biomass; Pretreatment; High solids; Simultaneous saccharification and fermentation (SSF); Cellulosic ethanol titer
Funding
- Woody Biomass, Bioenergy, and Bioproducts (WBBB)
- USFS-FPL
- Ministry of Science and Technology (MOST) of China
- USFS
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Native aspen (Populus tremuloides) was pretreated using sulfuric acid and sodium bisulfite (SPORL) and dilute sulfuric acid alone (DA). Simultaneous enzymatic saccharification and fermentation (SSF) was conducted at 18% solids using commercial enzymes with cellulase loadings ranging from 6 to 15 FPU/g glucan and Saccharomyces cerevisiae Y5. Compared with DA pretreatment, the SPORL pretreatment reduced the energy required for wood chip size-reduction, and reduced mixing energy of the resultant substrate for solid liquefaction. Approximately 60% more ethanol was produced from the solid SPORL substrate (211 L/ton wood at 59 g/L with SSF efficiency of 76%) than from the solid DA substrate (133 L/ton wood at 35 g/L with SSF efficiency 47%) at a cellulase loading of 10 FPU/g glucan after 120 h. When the cellulase loading was increased to 15 FPU/g glucan on the DA substrate, the ethanol yield still remained lower than the SPORL substrate at 10 FPU/g glucan. Published by Elsevier Ltd.
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