Journal
BIORESOURCE TECHNOLOGY
Volume 102, Issue 7, Pages 4633-4637Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.01.013
Keywords
Leachate recirculation; Enzyme augmentation; Lignin biodegradation; Peroxidase
Funding
- Natural Sciences and Engineering Research Council (NSERC) of Canada
- CEERE of University of Calgary
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Operation of waste cells as landfill bioreactors with leachate recirculation is known to accelerate waste degradation and landfill gas generation. However, waste degradation rates in landfill bioreactors decrease with time, with the accumulation of difficult to degrade materials, such as lignin-rich waste. Although, potential exists to modify the leachate quality to promote further degradation of such waste, very little information is available in literature. The objective of this study was to determine the viability of augmenting leachate with enzymes to increase the rate of degradation of lignin-rich waste materials. Among the enzymes evaluated MnP enzyme showed the best performance in terms of methane yield and substrate (lignin) utilization. Methane production of 200 mL CH4/g VS was observed for the MnP amended reactor as compared to 5.7 ml CH4/g VS for the control reactor. The lignin reduction in the MnP amended reactor and control reactor was 68.4% and 6.2%, respectively. (C) 2011 Elsevier Ltd. All rights reserved.
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