Journal
BIORESOURCE TECHNOLOGY
Volume 102, Issue 3, Pages 3564-3567Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2010.09.098
Keywords
Polyhydroxyalkanoate; Cellulose hydrolysate; Supported ruthenium catalyst
Funding
- New Energy and Industrial Technology Development Organization (NEDO)
- Japan Foundation for Applied Enzymology
- Japan Society for the Promotion of Science (JSPS) [20226016]
- Ministry of Education, Culture, Sports, Science and Technology, Japan
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Cellulose-derived glucose generated using the supported ruthenium catalyst was applied to poly(3-hydroxybutyrate) [P(3HB)] production in recombinant Escherichia coli. By the reaction with the catalyst at 220 degrees C. 15-20 carbon mol% of cellulose was converted into glucose. The hydrolysate also contained byproducts such as fructose, mannose, levoglucosan, oligomeric cellulose, 5-hydroxymethylfurfural (5-HMF), and furfural together with unidentified compounds. Setting the reaction temperature lower (215 degrees C) improved the ratio of glucose to 5-HMF, which was a main inhibiting factor for the cell growth. Indeed, the recombinant E. coli exhibited better performance on the hydrolysate generated at 215 degrees C and accumulated P(3HB) up to 42 wt%, which was the same as the case of the same concentration of analytical grade glucose. The result indicated that the ruthenium-mediated cellulose hydrolysis has a potency as a useful biorefinery process for production of bio-based plastic from cellulosic biomass. (C) 2010 Elsevier Ltd. All rights reserved.
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