Journal
BIORESOURCE TECHNOLOGY
Volume 102, Issue 20, Pages 9619-9625Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.07.055
Keywords
Escherichia coli O157:H7; Indigenous microorganisms; Compost; Dry-heat treatment; Denaturing gradient gel electrophoresis
Funding
- Center for Produce Safety
- UC Davis
- USDA NIFSI
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Both autoclaving and dry-heat treatments were applied to dairy manure-based compost to achieve target populations of indigenous microorganisms. A 3 strain-mixture of Escherichia coli O157:H7 of ca. 2 log CFU/g was inoculated into acclimated autoclaved compost (AAC) and dry heat-treated compost (DHTC) with different moistures, and stored at 8, 22, or 30 degrees C. Only selected groups of microorganisms grew in AAC during acclimation, whereas the relative ratio of each group of microorganisms was maintained in DHTC after heat treatment. E. colt O157:H7 grew more in MC than DHTC in the presence of same level of indigenous mesophiles. However, control compost (no heat treatment) did not support E. coli O157:H7 growth. Our results revealed that both the type and population of indigenous microorganisms is critical for suppressing E. coli O157:H7 growth in compost, and dry-heat treatment can result in a compost product which resembles cured compost with different levels of indigenous microorganisms. (C) 2011 Elsevier Ltd. All rights reserved.
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