Journal
BIORESOURCE TECHNOLOGY
Volume 102, Issue 3, Pages 3246-3253Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2010.11.034
Keywords
Cellulosic ethanol; Xylose isomerase; Simultaneous isomerization and fermentation (SIF) with native yeast; Co-immobilized enzymes; Mixed sugar fermentation (MSF)
Funding
- United States Department of Energy Office of the Biomass Program [GO18163]
- Ohio Third Frontier Advanced Energy Program [08-021]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [0933250] Funding Source: National Science Foundation
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In this paper, the feasibility of a technology for fermenting sugar mixtures representative of cellulosic biomass hydrolyzates with native industrial yeast strains is demonstrated. This paper explores the isomerization of xylose to xylulose using a bi-layered enzyme pellet system capable of sustaining a micro-environmental pH gradient. This ability allows for considerable flexibility in conducting the isomerization and fermentation steps. With this method, the isomerization and fermentation could be conducted sequentially, in fed-batch, or simultaneously to maximize utilization of both C5 and C6 sugars and ethanol yield. This system takes advantage of a pH-dependent complexation of xylulose with a supplemented additive to achieve up to 86% isomerization of xylose at fermentation conditions. Commercially-proven Saccharomyces cerevisiae strains from the corn-ethanol industry were used and shown to be very effective in implementation of the technology for ethanol production. (C) 2010 Elsevier Ltd. All rights reserved.
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