Journal
BIORESOURCE TECHNOLOGY
Volume 102, Issue 17, Pages 8130-8136Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.05.068
Keywords
Halomonas; Halophilic bacterium; PHB; Polyhydroxyalkanoates; Continuous fermentation
Funding
- National High Tech 863 Grants [2010AA101607]
- 973 Basic Research Fund [2007CB707807]
- Tsinghua University Initiative [2009THZ01005]
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An unsterile and continuous fermentation process was developed based on a halophilic bacterium termed Halomonas TD01 isolated from a salt lake in Xinjiang, China. The strain reached 80 g/L cell dry weight containing 80% poly(3-hydroxybutyrate) (PHB) on glucose salt medium during a 56 h fed-batch process. In a 14-day open unsterile and continuous process, the cells grew to an average of 40 g/L cell dry weight containing 60% PHB in the first fermentor with glucose salt medium. Continuous pumping of cultures from the first fermentor to the second fermentor containing the nitrogen-deficient glucose salt medium diluted the cells but allowed them to maintain a PHB level of between 65% and 70% of cell dry weight. Glucose to PHB conversions were between 20% and 30% in the first fermentor and above 50% in the second one. This unsterile and continuous fermentation process opens a new area for reducing the cost in polyhydroxyalkanoates production. (C) 2011 Elsevier Ltd. All rights reserved.
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