Journal
BIORESOURCE TECHNOLOGY
Volume 102, Issue 21, Pages 9919-9926Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.08.054
Keywords
Methanotrophic bacteria; Selection factor; Fluidized bed reactor; Polyhydroxybutyrate
Funding
- California Environmental Protection Agency [07T3451]
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Type II methanotrophs produce polyhydroxybutyrate (PHB), while Type I methanotrophs do not. A laboratory-scale fluidized bed reactor was initially inoculated with a Type II Methylocystis-like dominated culture. At elevated levels of dissolved oxygen (DO, 9 mg/L), pH of 6.2-6.5 with nitrate as the N-source, a Methylobacter-like Type I methanotroph became dominant within the biofilms which did not produce PHB. A shift to biofilms capable of PHB production was achieved by re-inoculating with Type II Methylosinus culture, providing dissolved N-2 as the N-source, and maintaining a low influent DO (2.0 mg/L). The resulting biofilms contained both Types I and II methanotrophs. Batch tests indicated that biofilm samples grown with N-2 became dominated by Type II methanotrophs and produced PHB. Enrichments with nitrate or ammonium were dominated by Type I methanotrophs without PHB production capability. The key selection factors favoring Type II were N-2 as N-source and low DO. (C) 2011 Elsevier Ltd. All rights reserved.
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