Journal
BIORESOURCE TECHNOLOGY
Volume 102, Issue 18, Pages 8432-8436Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2011.02.086
Keywords
Biohydrogen production; Hydrogen-producing bacterium; Clostridium beijerinckii; Isolation; Culture condition
Funding
- Program for New Century Excellent Talents in University [NCET-10-0066]
- National Natural Science Foundation of China [30870037, 30900046]
- State Key Laboratory of Urban Water Resource and Environment (Harbin Institute of Technology) [2010TS09]
- Foundation for Innovative Research Groups of the National Natural Science Foundation of China [50821002]
Ask authors/readers for more resources
A fermentative hydrogen-producing strain, RZF-1108, was isolated from a biohydrogen reactor, and identified as Clostridium beijerinckii on the basis of the 16S rRNA gene analysis and physiobiochemical characteristics. The effects of culture conditions on hydrogen production by C beijerinckii RZF-1108 were investigated in batch cultures. The hydrogen production and growth of strain RZF-1108 were highly dependent on temperature, initial pH and substrate concentration. Yeast extract was a favorable nitrogen source for hydrogen production and growth of RZF-1108. Hydrogen production corresponded to cell biomass yield in different culture conditions. The maximum hydrogen evolution, yield and production rate of 2209 ml H(2)/l medium. 1.97 mol H(2)/mol glucose and 104.20 ml H(2)/g CDW h(-1) were obtained at 9 g/l of glucose, initial pH of 7.0, inoculum volume of 8% and temperature of 35 degrees C, respectively. These results demonstrate that C. beijerinckii can efficiently produce H(2), and is another model microorganism for biohydrogen investigations. (C) 2011 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available