Journal
BIORESOURCE TECHNOLOGY
Volume 101, Issue 2, Pages 501-509Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2009.08.011
Keywords
Heavy metal-resistant-endophytic bacteria; Diversity; Characteristics; Elsholtzia splendens; Commelina communis
Funding
- Chinese National Natural Science Foundation [40371070, 40871127]
- Chinese National Programs for High Technology Research and Development [2006AA10Z404]
- 111 project [1307030]
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The diversity of endophytic bacteria from the copper-tolerant species Elsholtzia splendens and Commelina communis was evaluated by using cultivation and cultivation-independent techniques. Phylogenetic analysis based on 16S rDNA sequences showed that the Cu-resistant isolates belonged to three major phylogenetic groups: Firmicutes, Actinobacteria and Proteobacteria. Bacillus and Acinetobacter were predominant among the Cu-resistant isolates. Sequence analysis from the 16S rDNA clone libraries of the two plant roots revealed sequences of Alpha-, Beta-, Gamma-Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes. Gammaproteobacteria was predominant in the two 16S rDNA clone libraries. Thirty-two endophytic bacteria were isolated and characterized with respect to heavy metal resistance and production of plant growth-promoting factors. In experiments involving rape plants grown in vermiculite containing 4 mg kg(-1) of Cu, inoculation with the isolates was found to increase the dry weights of roots (ranging from 132% to 155%) and above-ground tissues (ranging from 71% to 83%) compared to the uninoculated control. Increase in above-ground tissue Cu content varied from 63% to 125% in inoculated-rape plants cultivated in Cu-contaminated substrate compared to the uninoculated control. (c) 2009 Elsevier Ltd. All rights reserved.
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